FIGURE

Fig. 3

ID

ZDB-FIG-201007-35

Publication

Yu et al., 2020 - Zebrafish Nedd8 facilitates ovarian development and the maintenance of female secondary sexual characteristics via suppression of androgen receptor activity

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Fig. 3

Loss of ar and/or treatment with the androgen antagonist flutamide eliminates BTs on the pectoral fins of nedd8−/− female zebrafish. (A) Images showing the effects of flutamide (Flu) treatment or ar knockout on the nedd8-knockout-induced BTs on the pectoral fins of female ar+/+nedd8−/− and ar+/−nedd8−/− zebrafish at 4 mpf. The red arrows indicate BTs. Panels on right show magnification of areas indicated by boxed areas on left. (B) Ratio of BT cluster length to pectoral fin ray length in ar+/+nedd8−/− (n=6) and ar+/−nedd8−/− (n=6) female zebrafish (4 mpf), plotted against pectoral fin ray number. (C) BTs/segment in ar+/+nedd8−/− (n=6) and ar+/−nedd8−/− (n=6) female zebrafish (4 mpf), treated with 2 mg/l flutamide for 11 days or untreated (control). (D) flk gene expression in the pectoral fins of nedd8+/+ (n=6) and nedd8−/− (n=6) female zebrafish (4 mpf) treated with 2 mg/l flutamide for 11 days or untreated (control). Gene expression was quantified using quantitative real-time PCR (qPCR). Data are mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 (unpaired Student's t-test).

Expression Data

Gene:	kdrl
Fish:	WT nedd8^{ihb1227/ihb1227}
Condition:	chemical treatment by environment: flutamide
Anatomical Term:	pectoral fin female organism
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT nedd8^{ihb1227/ihb1227} ar^ihb1225/+; nedd8^{ihb1227/ihb1227}
Condition:	chemical treatment by environment: flutamide
Observed In:	pectoral fin female organism pectoral fin breeding tubercle female organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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