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Impaired prey capture in six6a/six6b/six7 TKO zebrafish at 6 dpf. (A) Time-lapse images during a typical hunting episode. Paramecia are indicated by orange arrowheads. (Scale bar: 100 μm.) (B) Relative numbers of paramecia counted in the chamber containing WT and TKO larvae. The number of the paramecia left in the chamber was normalized to the initial count. The data are represented by mean ± SD (n = 12 for each genotype). (C) Relative numbers of paramecium at 10 min (mean ± SEM, n = 12; *P < 0.05, Student’s t test). (D) Distribution of eye vergence angle. The data are represented by mean ± SD (n = 12). (E) Frequency of eye convergence during 10 min. The eye convergence was defined as the state in which the eye vergence angle was more than 70°. A vergence angle greater than 70° is indicated by the bar in D. (F) Swimming speed of WT and TKO larvae. Values in E and F are presented as mean ± SEM (n = 12; *P < 0.05, Student’s t test).
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