FIGURE

Fig. 1

ID

ZDB-FIG-200922-12

Publication

Liu et al., 2020 - CFTR deficiency causes cardiac dysplasia during zebrafish embryogenesis and is associated with dilated cardiomyopathy

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Fig. 1

cftr mutation induces cardiac dysplasia in the early zebrafish embryo. (A) Lateral views of live zebrafish embryos at 60 hpf. Arrows indicate the pericardium. Heart rate (B) and quantification of ventricular fractional shortening (FS) (C) are represented in the scatter diagram. (D) The expression pattern of nkx2.5 at 13 hpf is significantly affected in cftr mutant embryos. Embryo orientations: dorsal view with anterior oriented at the top. Arrows indicate normal nkx2.5 expression; arrowheads demonstrate the aberrant strength and position of nkx2.5 expression in the cftr mutant; double-ended arrows indicate the distance between the two populations expressing nkx2.5. Relative expression of nkx2.5 (E) and relative distance of nkx2.5 lateral signal (F) are represented in the scatter diagram. (G) Heart morphology (marked by the pan-cardiomyocyte marker myl7) at 48 hpf was impaired significantly in cftr mutants. Embryo orientation: ventral view with the anterior at the top. Arrows point to the atrium; red lines demonstrate the acute angle formed between the atrial and ventricular axes. Looping angle (H) and area ratio of atrium/ventricle (I) are represented in the scatter diagram. The expression of bmp4 (J and K) and has2 (L and M) is dramatically reduced in cftr mutant embryos at 48 hpf. Embryo orientation: ventral views with the anterior at the top. Arrows point to the signal generated by detected marker genes.

Expression Data

Genes:	bmp4 has2 myl7 nkx2.5
Fish:	WT cftr^{scu101/scu101}
Anatomical Terms:	cardiac muscle cell heart lateral mesoderm whole organism
Stage Range:	5-9 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cftr^{scu101/scu101}
Observed In:	atrium heart heart contraction heart looping lateral mesoderm whole organism
Stage Range:	5-9 somites to Pec-fin

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 163, Liu, Y., Lin, Z., Liu, M., Liao, H., Chen, Y., Zhang, X., Chan, H.C., Zhou, B., Rao, L., Sun, H., CFTR deficiency causes cardiac dysplasia during zebrafish embryogenesis and is associated with dilated cardiomyopathy, 103627, Copyright (2020) with permission from Elsevier. Full text @ Mech. Dev.