FIGURE

Figure 5.

ID

ZDB-FIG-200829-68

Publication

Liu et al., 2020 - TMEM216 Deletion Causes Mislocalization of Cone Opsin and Rhodopsin and Photoreceptor Degeneration in Zebrafish

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Figure 5.

Rod outer segments were reduced in tmem216 knockout zebrafish. Retinal sections were stained with rhodopsin marker, 4D2 (red), and counterstained with DAPI to label nuclei. Knockout images shown represent the tmem216^snyΔ175 retina, similar phenotypes were observed in the tmem216^snyR8Δ60 homozygous retina. (A–F) Wildtype at 3-, 7-, and 14-dpf, respectively. (G–L) tmem216^snyΔ175 homozygous at 3-, 7-, and 14-dpf, respectively. (M–O) 4D2 immunofluorescence intensity was significantly reduced at 3-, 7-, and 14-dpf in tmem216^snyΔ175 homozygous retina (n = 3, Student's t-test). Scale bar in L: 55 µm for A and G; 110 µm for C, I, E, and K; 6 µm for B, H, D, J, F, and L.

Expression Data

Antibody:	Ab5-rho
Fish:	AB/TU tmem216^{zf3394/zf3394} (AB/TU) tmem216^{zf3395/zf3395} (AB/TU)
Anatomical Term:	retinal rod cell rod photoreceptor outer segment
Stage Range:	Protruding-mouth to Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tmem216^{zf3394/zf3394} (AB/TU) tmem216^{zf3395/zf3395} (AB/TU)
Observed In:	retinal rod cell rod photoreceptor outer segment
Stage Range:	Protruding-mouth to Days 14-20

Phenotype Detail

Acknowledgments

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