FIGURE

Fig. 5

ID

ZDB-FIG-200723-25

Publication

Vonica et al., 2020 - Apcdd1 is a dual BMP/Wnt inhibitor in the developing nervous system and skin

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Fig. 5

Expression of zebrafish apcdd1 and effects of functional knockdown on embryonic patterning. A-G. Domains of expression of various markers of gastrula-stage embryos. Expression of chd in the organizer and vent in ventrolateral mesoderm (A, B, animal pole view) and dkk1 in the organizer (C, lateral view). The organizer is expanded laterally at 6 hpf in apcdd1l morphants, and there is a corresponding contraction of ventrolateral tissue. At 10 hpf, expression of brachyury-a (ta) in the notochord (D) and otx2b in the anterior neural plate (E) (dorsal views, anterior up) are expanded in apcdd1l morphants, whereas expression of ventral marker tp63 in epidermal ectoderm (G, lateral view) is contracted. At this time, expression of bmp4 at the ventral midline (F, dorsal view) begins to upregulate and expand in apcdd1l morphants, presaging subsequent ventralization. At least n = 15 zebrafish embryos were examined for each time point and experimental condition.

Expression Data

Genes:	bmp4 chrd dkk1b otx2b tbxta tp63 vent
Fish:	WT WT + MO1-apcdd1l + MO4-tp53
Knockdown Reagents:	MO1-apcdd1l MO4-tp53
Anatomical Terms:	anatomical structure ectoderm neuroectoderm notochord shield whole organism ventro-lateral region
Stage Range:	Shield to Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-apcdd1l + MO4-tp53
Knockdown Reagents:	MO1-apcdd1l MO4-tp53
Observed In:	anatomical structure ectoderm neuroectoderm notochord shield whole organism ventro-lateral region
Stage Range:	Shield to Bud

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 464(1), Vonica, A., Bhat, N., Phan, K., Guo, J., Iancu, L., Weber, J.A., Karger, A., Cain, J.W., Wang, E.C.E., DeStefano, G.M., O'Donnell-Luria, A.H., Christiano, A.M., Riley, B., Butler, S.J., Luria, V., Apcdd1 is a dual BMP/Wnt inhibitor in the developing nervous system and skin, 71-87, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.