Gill rakers and food intake of zebrafish with activin A and BMP4 treatment.a Number of zebrafish gill rakers (arrowheads, 0.1% alcian blue staining) at 2, 3 and 8 dph. b Zebrafish edar expression assessed by whole-mount in situ hybridization. Control fish or fish treated with 1 ng/ml activin A or 50 ng/ml BMP4 at 2, 3, 8 and dph and the negative control (N-Ctrl) are shown. c Zebrafish larvae intake of brine shrimp. d The distance between gill rakers of zebrafish at 8 dph (n = 3). e Zebrafish feeding at 8 dph. The numbers of fish that ingested 5-day-old shrimp were shown (n = 20). The boxplot elements are defined as: center line, median; box limits, the third and first quartiles; whiskers, 1.5× interquartile range. * indicates a significant difference (P < 0.05).
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