Fig. 1

Inhibition of Cx43-GJIC recapitulates sofb123 phenotypes. (A) Schematic of Cx43 showing the location of Gap27 interaction with the second extracellular loop. N, amino terminus; C, carboxy terminus; EL1/EL2, extracellular loop1/2; CL, cytoplasmic loop. (B) Representative longitudinal cross-sections showing localization of 5FAM-Gap27 (green) following its injection. DAPI (blue) stains cell nuclei. m, mesenchyme; e, epidermis. UN, uninjected. (C) Regenerate length is reduced in Gap27-injected fins compared with Scrm-Gap27-injected fins. All fins were amputated at the 50% level. The amputation plane is indicated (black line). Black arrow indicates the distance from the amputation plane to the distal tip of the third fin ray. Injections were performed at 3?dpa and measurements were taken at 7?dpa. UN, uninjected. Bar graph (F) shows a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27 (one-way ANOVA with Tukey's and Dunnett's multiple comparison as post-hoc tests; *P<0.05). n=21; three independent replicates. (D) Calcein-stained fin rays show reduced segment length in Gap27-injected fins compared with Scrm-Gap27-injected fins. Arrows and arrowheads point to the first two joints following the amputation plane (white line). The inset shows a higher magnification of the segments. Bar graph (G) reveals a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27 (one-way ANOVA with Tukey's and Dunnett's multiple comparison as post-hoc tests; *P<0.05). n=19; three independent replicates. (E) H3P+ cells are reduced in number in Gap27-injected fins compared with Scrm-Gap27-injected fins. Arrowheads identify one H3P+ cell per image. Bar graph (H) reveals a significant reduction in the percentage similarity between Gap27 and Scrm-Gap27. Student's t-test, two tailed and unpaired, *P<0.05 (n=23, three independent replicates). Data are mean±s.e.m.