Fig. 3

EdU incorporation is reduced in the hearts of udu?/? embryos. Experimental workflow for EdU labeling (A). EdU labeling (red) in 48 hpf WT hearts (B) and udu?/? hearts (C) with cardiomyocytes labeled by Mef2 (blue). Arrows showing EdU ?+ ?cardiomyocyte (white) and non-cardiomyocyte (green). Graph showing the proliferative index for EdU in WT (grey) and udu?/?(blue) cardiomyocytes (D). qRT-PCR for ccnd1 (E), ccnd2a (F), ccnd2b (G), ccnd3 (H), ccne1 (I), and ccne2 (J) performed on RNA extracted from hearts isolated from 36 hpf WT (grey) and udu?/?(blue) embryos. Results are shown as fold change of gene expression in udu?/? embryos compared to WT and results were standardized to gapdh expression. DamID-seq Genome browser tracks of Gon4l-Dam and GFP Control-Dam at ccnd1 (E?), ccnd2a (F?), ccnd2b (G?), ccnd3 (H?), ccne1 (I?), and ccne2 (J?) loci. ?p ?< ?0.05, ??p ?< ?0.01, ???p ?< ?0.001, ????p ?< ?0.0001, error bars ?= ?SEM. Scale bar represents 50 ??m.