FIGURE

Fig. 3

ID

ZDB-FIG-200528-1

Publication

Tamaoki et al., 2020 - Splicing- and demethylase-independent functions of LSD1 in zebrafish primitive hematopoiesis

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Fig. 3

Phenotypic rescue experiments using human and zebrafish LSD1 variants. (A) The gata1a expression at 15 hpf in the lateral plate mesoderm (arrowheads) of wild-type or kdm1a^it627 embryos injected with or without mRNAs encoding indicated human LSD1 variants was detected by WISH analysis. +2a, LSD1+2a; +8a, LSD1+8a; +2a8a, LSD1+2a8a. The numbers in each picture indicate the numbers of embryos with similar staining profiles and tested embryos. WISH was performed in at least two separated trials. (B) Immunoblot analysis using anti-FLAG antibody of FLAG-tagged LSD1 variants in 15-hpf wild-type embryos injected with or without mRNAs encoding indicated human LSD1 variants. Total protein loading was shown by CBB staining. (C) The gata1a expression at 15 hpf in the lateral plate mesoderm (arrowheads) of wild-type or kdm1a^it627 embryos injected with or without mRNAs encoding indicated zebrafish LSD1 variants. +2al, LSD1+2al; +8al, LSD1+8al; +2a8al, LSD1+2a8a. (D) Immunoblot analysis using anti-FLAG antibody of FLAG-tagged LSD1 variants in 15-hpf wild-type embryos.

Expression Data

Gene:	gata1a
Fish:	AB kdm1a^it627/it627 (AB)
Anatomical Term:	lateral plate mesoderm
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kdm1a^it627/it627 (AB)
Observed In:	lateral plate mesoderm
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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