Fig. 2

a Bacterial burdens of larvae injected with different CFU doses of Mm that have been analyzed by RNAseq. The data are accumulated from three independent sample sets at 4 dpi (>42 larvae/group) and represented by scatter and boxplots as detailed in the methods section. b Venn diagram of the significantly enriched KEGG pathways in the transcriptome of larvae infected with Mm. The enrichment comparisons were performed on dram1^{∆19n/∆19n} 150 CFU versus dram1^{∆19n/∆19n} PBS, dram1^{∆19n/∆19n} 300 CFU versus dram1^{∆19n/∆19n}, and dram1^+/+ 300 CFU versus dram1^+/+ PBS. c Visualization of the lytic cellular death signaling pathway transcriptome shows different responses in the transcriptome of infected dram1^{∆19n/∆19n} and dram1^+/+. The pathway was adapted from the KEGG pathway necroptosis. In the visualization, the gene expression in the comparison dram1^{∆19n/∆19n} 150 CFU versus dram1^{∆19n/∆19n} PBS, dram1^{∆19n/∆19n} 300 CFU versus dram1^{∆19n/∆19n}, and dram1^+/+ 300 CFU versus dram1^+/+ PBS are depicted by color gradient (yellow, upregulated, blue downregulated). The expression of all genes of the pathway present in the RNA sequencing was plotted independently of their significance. While the effector genes of the apoptosis pathway did not show high expression changes, the genes from lytic cell death forms, including pyroptosis, showed high expression modulation.