Figure 3

Valproate treatment. (A) Valproate (2 mM) was administered to MO kcnj10a larvae after 30 min of baseline recording of LFP and two-photon imaging. After a diffusion time of 15 min, the double recording was resumed. (B) Alignment of LFP and ΔF/F0 calcium traces in MO VPA( Valproic Acid)-treated larvae. (C) The analysis of the statistical distribution of the electrophysiological signal showed a clear effect of VPA in decreasing the energy of the LFP events. (D) The skewness of statistical distributions of Ca²⁺ fluctuations decreased during VPA treatment. (E) During VPA treatment, movements of the larvae did not correlate with a generalized increase of calcium signal. Therefore, VPA treatment led to a physiological phenotype, where tail flicks are associated not to a convulsive seizure but to the activation of a limited neuronal circuitry. 2 recordings for each larva, n = 3 larvae for each condition (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).