FIGURE

Figure 7.

ID

ZDB-FIG-200423-116

Publication

Lim et al., 2020 - Application of optogenetic Amyloid-β distinguishes between metabolic and physical damages in neurodegeneration

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Figure 7.

HEK cells expressing Aβ-CRY2-mCh in light condition.

(a) Untreated control HEK cells show light-induced clustering of Aβ-CRY2-mCh and increased number of vacuoles under blue light illumination. (b) HEK cells treated with GSK3 inhibitor CHIR99021 (positive control) show little clustering of the light-induced Aβ-CRY2-mCh. Fewer cellular vacuoles observed. (c) HEK cells treated with lithium chloride show homogenous expression of Aβ-CRY2-mCh, whereby blue light illumination did not result in significant clustering of Aβ-CRY2-mCh. (d) Quantification of cluster number per cell showed significantly reduced cluster formation in cells treated with CHIR99021 and lithium chloride as compared to control cells. (**p<0.01) (e) Quantification of the mean intensity of the clusters showed significantly reduced intensity of the clusters when the cells were treated with GSK3 inhibitor CHIR99021 and lithium chloride as compared to control cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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