FIGURE

Fig. 5

ID

ZDB-FIG-200413-51

Publication

Chestnut et al., 2019 - Zebrafish etv2 knock-in line labels vascular endothelial and blood progenitor cells

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Fig. 5

A comparison of etv2 ci32Gt+/?; UAS:GFP, TgBAC(etv2:GFP), Tg(?2.3etv2:GFP) fluorescence pattern (in kdrl:mCherry background) at 48 hpf, A?I, and 72 hpf, J?R. etv2 ci32Gt+/?; UAS:GFP expression is apparent throughout the entire vasculature and in lymphatic progenitors (parachordal lymphangioblasts, PLs). TgBAC(etv2:GFP) shows similar expression pattern, and also shows nonspecific expression in the neural tube. Vascular endothelial expression in Tg(?2.3etv2:GFP) line is downregulated after 48 hpf and is very weak at 72 hpf, while nonspecific epithelial expression is apparent. A?C, J?L, Merged mCherry and GFP channels; D?F, M?O, GFP channel; G?I, P?R, magnified images of the trunk region in, A?C, J?L. DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessels, SIV, subintestinal vessel; DLAV, dorsal longitudinal anastomotic vessel; CCV, common cardinal vein

Expression Data

Genes:	EGFP mCherry
Fish:	ci1Tg; ci5Tg ci5Tg; zf372Tg etsrp^ci32Gt/+; ci5Tg/+; nkuasgfp1aTg
Anatomical Terms:	blood vasculature blood vessel endothelial cell common cardinal vein cranial vasculature dorsal aorta dorsal longitudinal anastomotic vessel endothelial cell intersegmental vessel lymphangioblast cord notochord posterior cardinal vein subintestinal vein thoracic duct
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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