FIGURE

Fig. 1

ID

ZDB-FIG-200325-48

Publication

Ren et al., 2020 - Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish

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Fig. 1

Generation and characterization of <italic>igf2bp3</italic> mutant zebrafish.

a Design and mutation types of igf2bp3 based on CRISPR/Cas9 biotechnology. Exons were represented by purple boxes and the sgRNA target sequence was shown in red. Two types of mutants were generated. b Illustration of deduced protein structure of wild-type igf2bp3 and two mutated igf2bp3. c The developmental timecourse of wild type and Migf2bp3 embryos. Migf2bp3 embryos displayed an inapparent septum (white arrow) compared to that in wild type (black arrow) at 8-cell stage. The non-adhesive cells in the mutants were indicated by red arrows at 32- and 512-cell stage. d Statistical analysis of the phenotypes of Migf2bp3 and wild-type zebrafish embryos at 512-cell stage, as indicated in c. Wild type, N = 5, n = 708; Migf2bp3 (∆2nt), N = 6, n = 672; Migf2bp3 (∆41nt), N = 8, n = 749 (N = female individuals, n = embryos). Scale bars: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	igf2bp3^hza57/hza57 (AB) igf2bp3^hza58/hza58 (AB)
Observed In:	blastodisc cleavage furrow blastomere cleavage furrow ingression embryonic cleavage fertilized egg yolk
Stage Range:	1-cell to 512-cell

Phenotype Detail

Acknowledgments

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