Fig. 1-S1

( a) Expression patterns of a number of feeding-related peptides in the zebrafish hypothalamus, based on antibody-staining or transgenic labels (see Materials and methods). HCRT = hypocretin (orexin), CART = cocaine and amphetamine related transcript MCH = melanin concentrating hormone, TH = tyrosine hydroxylase (labels dopaminergic and/or noradrenergic neurons), MSH = alpha melanocyte stimulating hormone, AgRP = Agouti related peptide, NPY = neuropeptide Y, VMAT = vesicular monoamine transporter (labels dopaminergic (DA) and serotonergic neurons (5-HT)). Note that MCH and HCRT staining is absent from the zebrafish LH. Though not apparent from the schematic, HCRT is located more dorsally. The preoptic area, which contains oxytocinergic as well as other peptidergic neurons, is located more dorsally and not reflected in this schematic. ( b) Schematic view from the ventral perspective summarizing zebrafish hypothalamic peptide expression. GABA (dark blue) and glutamatergic (blue) neurons are found in the zebrafish LH (see Figure 1—figure supplement 2) and also throughout the medial regions of the hypothalamus. PVO = paraventricular organ, which also contains DA and 5-HT neurons. A number of peptidergic neurons are located within the anterior and posterior pituitary/hypophysis (aPit and pPit). Color code corresponds to images in ( a). A = anterior, R = right.