FIGURE

Figure 5

ID

ZDB-FIG-200314-72

Publication

Kang et al., 2020 - Global Transcriptomic Analysis of Zebrafish Glucagon Receptor Mutant Reveals Its Regulated Metabolic Network

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Figure 5

GCGR regulates carbohydrate metabolism in zebrafish larvae. (A) Heatmaps of transcripts in carbohydrate metabolism enrichment. Colors represent high (red), low (blue), or average (white) expression values based on the Z-score normalized FPKM values for each gene. The Z-score indicators are shown under each map. The fold change (log2) are shown on the right. (B,C) 2-NBDG glucose uptake of wildtype (B) and gcgr^−/− mutant (C) larvae, the glucose uptake level is indicated by the fluorescence of lens (arrow) imaged by fluorescent microscopy. (D–E) Representative images of the principal islet of gcgr^−/−; Tg(gcga:GFP) (D) and 20 mM glucose treated-gcgr^−/−; Tg(gcga:GFP) (E). The images are confocal projections; scale bars indicate 10 μm. (F) Quantification of the α-cell number in different groups of zebrafish at 7 dpf, n ≥ 10. Ns: No significance, and ** p < 0.01 by t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gcgra^vu600/vu600; gcgrb^vu601/vu601 gcgra^vu600/vu600; gcgrb^vu601/vu601; ia1Tg
Condition:	chemical treatment: glucose
Observed In:	D-glucose import pancreatic A cell
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

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