FIGURE

Figure 4

ID

ZDB-FIG-200314-57

Publication

Rojas-Benítez et al., 2020 - Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development

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Figure 4

Shh activity is diminished in ELP morphants. (A) Immunohistochemistry usnig an anti-Engrailed antibody to visualize the adaxial cells. Control, elp3 morphants and MO/PKA^DN mRNA-injected animals were analyzed 24 hpf. (B) The number of engrailed(+) cells were counted (N = 4, n = 30, ANOVA *** p < 0.005). (C) In situ hybridization to detect the expression pattern of col2a1 in control, elp3 morphants and MO/PKA^DN mRNA-injected fish (images representative of four independent experiments). Scale bar 40 µm.

Expression Data

Gene:	col2a1a
Antibody:	Ab1-eng
Fish:	WT WT + MO1-elp3 WT + MO2-elp3
Knockdown Reagents:	MO1-elp3 MO2-elp3
Anatomical Terms:	adaxial cell notochord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-elp3 WT + MO2-elp3
Knockdown Reagents:	MO1-elp3 MO2-elp3
Observed In:	adaxial cell notochord
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.