FIGURE

Figure EV4

ID

ZDB-FIG-200212-41

Publication

Ki et al., 2019 - CEP41-mediated ciliary tubulin glutamylation drives angiogenesis through AURKA-dependent deciliation

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Figure EV4

Hypoxia induces ciliary tubulin glutamylation and cilia disassembly in <styled-content toggle='no' style='fixed-case'>HUVEC</styled-content>s

HUVECs were cultivated under either normoxia or hypoxia (short and long exposure) conditions and were immunostained with ARL13b‐ and GT335‐specific antibodies. Representative images indicated with rectangles appear in magnified images in the lower panels. Scale bars, 20 μm.

B–E

Quantification of the numbers of ciliated cells (B), ciliary length (C, D), and relative intensity of GT335/ARL13b signals in the cilia (E) in images (A) is the result of three independent experiments with ≥ 200 cells per condition (mean ± SD or median). **P < 0.001, ***P < 0.001, ns: non‐significant (one‐way ANOVA with Dunnett's post hoc test (B, D) and Brown–Forsythe ANOVA with Dunnett's T3 post hoc test (C) and Kruskal–Wallis test with Dunn's post hoc test (E)).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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