FIGURE

Figure 6

ID

ZDB-FIG-200124-9

Publication

Gramann et al., 2019 - Regulation of zebrafish melanocyte development by ligand-dependent BMP signaling

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Figure 6

BMP inhibition impacts fate specification of mitfa-positive pigment progenitor cells. (A) Diagram of lineage tracing experiment. Embryos containing Tg(ubi:switch) were injected with a mitfa:Cre-ERT2 construct and treated with BMPi and tamoxifen (4-OHT) from 12 to 24 HPF to block BMP signaling and allow Cre recombination. At 5 DPF, animals were screened for successful recombination by presence of single mCherry-labeled pigment cells, and the identities of those cells were assessed using incident light. Scale bar = 40 µm. (B) Quantification of mCherry-labeled cell fates at 5 DPF in vehicle and BMPi-treated animals, n = 101 and 80 labeled cells for vehicle and BMPi groups, respectively, from five independent experiments (N = 5); P-value calculated using Fisher’s exact test, *p<0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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