Receptor internalization limits neutrophil motion at wounds. a Confocal projections of neutrophil distribution in Tg(lyz:Cxcr1-WT-FT)/cxcr1−/− larvae (WT), Tg(lyz:Cxcr1-ala-FT)/cxcr1−/− (ala), Tg(lyz:Cxcr1-chim-FT)/cxcr1−/−(chim) at ~2 hpw. Dashed line indicates occupied wound area (owa). CHT: caudal hematopoietic tissue, VF: ventral fin, W: wound. Scale bar = 32 µm. b Quantification of neutrophil cluster size, n = 8 (WT), n = 6 (ala), and n = 4 (chim) larvae from 3 imaging sessions per condition. One-way ANOVA test with Tukeyʼs multiple comparisons test. c Quantification of speed within the owa. n = 9 (WT), n = 6 (ala), and n = 7 (chim) larvae. One-way ANOVA test with Tukeyʼs multiple comparisons test. d Neutrophil speed in relation to distance from the owa. Average speeds per cell step per distance bin are shown. n = 316–1942 steps per bin (WT), n = 105–706 steps per bin (ala), n = 83–896 steps per bin (chim). e Neutrophil speed in relation to cosine of angle θ. Average speeds per cell per cosθ bin are shown. n = 128–849 steps per bin (WT), n = 22–445 steps per bin (ala), and n = 44–417 steps per bin (chim). f Net reverse traffic. n = 9 (WT), n = 6 (ala), and N = 7 (chim) larvae. Kruskal–Wallis test with Dunn’s multiple comparisons test. g Summary of phenotypes observed in Cxcr1 rescue experiments and their interpretation. PM, plasma membrane. For c–f, data are from 9 (WT), 6 (ala), and 7 (chim) larvae from 3, 6, and 3 imaging sessions, respectively. For all panels, analysis was focused on the post initial arrival window of 1–2 hpw. Error bars represent S.E.M. across cell steps (d,e) or larvae (b,c,f). Source data are provided as a Source Data file
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