FIGURE

Figure 5

ID

ZDB-FIG-191230-385

Publication

Cayuso et al., 2019 - Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness

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Figure 5

Taz and Tead1a are required for boundary expression of rfng. (A–H) Hindbrain boundary expression of rfng is reduced in taz knockdowns (B), and in taz (C) and tead1a (F) transient knockouts compared to controls (A), while yap1 knockdown (D) and yap1 (E) and tead3a (G) transient knockouts have normal rfng expression. (H) Scoring of boundary expression of rfng in different conditions according to severity levels: wild type = normal expression of rfng in all boundaries; severity 1 = general reduction of rfng expression levels; severity 2 = partial absence of rfng expression leading to discontinuous boundaries; severity 3 = total absence of rfng boundary expression. Number of embryos: control (15); MO-taz (20); CRISPR-taz (30); MO-yap1 (52); CRISPR-yap1 (21); CRISPR-tead1a (23); CRISPR-tead3a (12). Dorsal views, anterior to the top. Scale bar: 50 μm.

Expression Data

Gene:	rfng
Fish:	WT WT + MO1-wwtr1 WT + MO1-yap1
Knockdown Reagents:	MO1-wwtr1 MO1-yap1
Anatomical Terms:	rhombomere 1 posterior margin rhombomere 2 posterior margin rhombomere 3 posterior margin rhombomere 4 posterior margin rhombomere 5 posterior margin rhombomere 6 posterior margin
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wwtr1
Knockdown Reagent:	MO1-wwtr1
Observed In:	rhombomere 1 posterior margin rhombomere 2 posterior margin rhombomere 3 posterior margin rhombomere 4 posterior margin rhombomere 5 posterior margin rhombomere 6 posterior margin
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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