Fig. 3

Quantification of bacterial burden and survival after M. marinum infection. tlr2^+/+ (a), tlr2^+/− (b) and tlr2^−/− (c) embryos were infected with mCherry-labeled M. marinum strain Mma20 at a dose of ~ 150 CFU by caudal vein infection at 28 hpf. Representative images for bacterial pixel count in tlr2^+/+ (a), tlr2^+/− (b) and tlr2^−/− (c) were taken at 4 dpi. Bacterial burden of tlr2^+/+, tlr2^+/− and tlr2^−/− were also quantified at 3 dpi (d) and 4 dpi (e). Bacterial burdens were quantified by using bacterial fluorescence pixels. Red stars in (e) indicate the data for the representative images shown in (a-c). In (d, e), data (mean ± SEM) were combined from two independent experiments. Statistical significance of differences was determined by one-way ANOVA with Tukey’s Multiple Comparison method as a post-hoc test for comparison between more than two groups (d, e). Percent of survival curves for tlr2^+/+ (n = 47), tlr2^+/− (n = 49) and tlr2^−/− (n = 49) (f) are based on two independent experiments. Statistical significance of difference was determined by a log-rank (Mantel-Cox) test. ns, non-significant; *, P < 0.05, **, P < 0.01, ****, P < 0.0001