FIGURE

Figure 5—figure supplement 1.

ID

ZDB-FIG-191230-1600

Publication

Young et al., 2019 - Developmentally regulated Tcf7l2 splice variants mediate transcriptional repressor functions during eye formation

Other Figures

All Figure Page

Back to All Figure Page

Figure 5—figure supplement 1.

4L-Tcf7l2 and 45L-Tcf7l2 variants interact with β-Catenin and Tle3b in yeast two-hybrid protein interaction assays.

Y2Gold yeast strain was co-transformed with: (1) β-catenin/pGAD and empty pGBK vector, (2) β-catenin/pGAD and 4L-tcf7l2/pGBK (A, B) or 45L-tcf7l2/pGBK (C, D) (3) empty pGAD vector and 4L-tcf7l2/pGBK (A, B) or 45L-tcf7l2/pGBK (C, D) with, (4) dCtle3b/pGAD and Empty pGBK vector, (5) dCtle3b/pGAD and 4L-tcf7l2/pGBK (A, B) or 45L-tcf7l2/pGBK (C, D) (6) empty pGBK and pGAD vectors. and plated in -Leu-Trp dropout selective media agar plates supplemented with Xgal (A, C) or Ade-His-Leu-Trp dropout selective media agar plates supplemented with Aureoblastidin A and X-gal (B, D).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife