Loss of vmp1 in zebrafish causes accumulation of neutral lipids in the intestine and liver. (A) Oil red O and hematoxylin staining of 6-dpf vmp1+/- and vmp1-/- zebrafish. Data are representative of two independent experiments. Scale bars, 100 μm. (B) and (C) Transmission electron microscopy of the hindbrain (B) and skeletal muscle (C) from 6-dpf vmp1+/- and vmp1-/- zebrafish. Data are representative of two independent experiments. Scale bars, 1 μm. (D) and (E) Transmission electron microscopy of the intestine from 6-dpf rb1cc1+/-, rb1cc1-/- (D), atg5+/- and atg5-/- (E) zebrafish. L, lumen of the intestine. Data are representative of two independent experiments. Scale bars, 5 μm.
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