FIGURE

FIGURE 7

ID

ZDB-FIG-191230-1142

Publication

Huang et al., 2019 - BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development

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FIGURE 7

Expression of GDNF in the intestinal mesenchyme requires BMP2 signaling. (A,a,b) control embryos(97/103, 94.2%), (A,c,d) BMP 2b MO treated embryos) lateral views of 72 hpf whole mount embryos(112/124, 90.3%) that have been hybridized with a gdnf antisense probe. Compared with the control group, there is only partial expression of GDNF at the anterior end of the intestine at this age. (A,e,f) BMP2b MO plus heat-shocked at 10 hpf(84/105, 80%), the expression of GDNF was partly rescued. (A,j,h) BMP2b MO plus BMP2b mRNA were co-injected, the expression of GDNF was partly rescued(84/105, 80%). (B) The intensity of GDNF expression in different group. The expression of GDNF mRNA in the BMP2b MO group is approximately 2.7 fold the intensity of BMP2b MO + HS group. The expression of GDNF mRNA in the BMP2b MO group is approximately 2.5 fold the intensity of BMP2b MO + BMP2b mRNA group. Values are given as mean ± SE. n = 6, t-test, ^∗P < 0.05, compared with BMP2b MO + HS group or BMP2b MO + BMP2b mRNA group.) (C) Real time PCR analysis of GDNF mRNA expression. The GDNF mRNA expression was rescued in BMP2b + HS group, n = 6, t-test, ^∗P < 0.05, compared with BMP2b MO group. Bar, 100 μm.

Expression Data

Gene:	gdnfa
Fish:	WT WT + MO5-bmp2b fr13Tg + MO5-bmp2b
Condition:	heat shock
Knockdown Reagent:	MO5-bmp2b
Anatomical Term:	posterior intestine
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO5-bmp2b fr13Tg + MO5-bmp2b
Condition:	heat shock
Knockdown Reagent:	MO5-bmp2b
Observed In:	posterior intestine
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.