Fig. S7

Effects of overexpression of N2ICD or N3ICD on MC markers expression. (A) The 27 hpf Tg(TP1:H2B-mCherry) embryos injected with a plasmid encoding CMV:GFP-N2ICD (right) or uninjected control (left). Overexpression of GFP-N2ICD enhanced TP1:H2B-mCherry expression especially around yolk sac. (B) Confocal stack images of the 27 hpf Tg(TP1:H2B-mCherry) embryos injected with a plasmid encoding CMV:GFP-N2ICD. Boxed regions are enlarged below. In yolk tube, all GFP-N2ICD positive Development: doi:10.1242/dev.165589: Supplementary information Development • Supplementary information cells were also positive for TP1:H2B-mCherry (a). While, some N2ICD-GFP positive cells were also positive for TP1:H2B-mCherry (arrows) but some of them were negative for the TP1:H2B-mCherry (arrowheads) in the trunk (b). This data may suggest the differences of the sensitivity to N2ICD among the cell types. (C) Confocal stack images of the 29 hpf or 50 hpf TgBAC(pdgfrb:mCherry) embryos injected with a plasmid encoding CMV:GFP-N2ICD. Note that GFP-N2ICD could not induce pdgfrb:mCherry expression in any body parts including yolk sac even though GFP-N2ICD clearly enhanced TP1:H2B-mCherry expression, as shown in A and B. (D, E) Confocal stack images of the 29 hpf, 33 hpf, or 50 hpf TgBAC(pdgfrb:mCherry) embryos injected with a plasmid encoding CMV:GFP-N3ICD (D) or GFP-N3ICD mRNA (E). N3ICD could not induce pdgfrb:mCherry expression. (F, G) Confocal stack images of the 50 hpf TgBAC(abcc9:GAL4FF);Tg(UAS:GFP) (F) or TgBAC(tagln:GFP) embryos (G) injected with a plasmid encoding CMV:mCherry-N2ICD. N2ICD could not induce abcc9- nor tagln-reporter expression