FIGURE

Fig. 3

ID

ZDB-FIG-191007-2

Publication

Grassini et al., 2019 - Myosin Vb is required for correct trafficking of N-cadherin and cardiac chamber ballooning

Other Figures

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Fig. 3

myo5b mutant cardiomyocytes have increased recycling endosomes. A: Electron microscopic images of 36 hpf sibling and uq^23ks mutant cardiomyocytes showing nonmitochondrial vesicles and tubular structures (blue arrows). M, mitochondria; Scale bar = 2.5 μm. B: Graph quantifying vesicles and tubular structures in siblings vs. mutant cardiomyocytes within 1 μm of the plasma membrane; mean ± SEM. C: Confocal imaging of recycling endosomes (labeled with RFP‐Rab11a) in sibling and mutant cardiomyocytes at 48 hpf. Cardiomyocyte membranes demarcated with white outline. D: Dot plot of the number of recycling endosomes per cell (as determined by RFP‐Rab11a‐postive particles), showing significantly more recycling endosomes in cardiomyocytes of uq^23ks mutants vs. siblings. E: Histograms of recycling endosome size, showing significantly larger endosome size, consistent with endosome fusion.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myo5b^{uq23ks/uq23ks}
Observed In:	cardiac muscle cell cardiac muscle cell endocytic recycling cardiac muscle cell recycling endosome
Stage Range:	Prim-25 to Long-pec

Phenotype Detail

Acknowledgments

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