Fig. 6

Nidogen is required during retinal development. A) Nidogen 1a and 1b morpholino injection phenotypes at various concentrations and combinations. Moderate phenotypes (gray bar) included potential developmental delays but lacked overt morphological deficiencies. Severe phenotypes (black bar) included significant developmental delays and morphological deficiencies. Lethal phenotypes (red bar) included monster embryos and major developmental deficiencies. B)WISH for nid2a comparing expression in WT and nid2a^sa15280 −/− embryos at 24hpf. Nid2a signal is completely absent in nid2a^sa15280 −/− embryos. C) Nidogen 1a and 1b morpholino injections into nid2a^sa15280−/− embryos. Sub-effective doses of nid1a and nid1b MOs, 0.5 or 0.75 ng each, resulted in up to 80% of embryos exhibiting severe or lethal phenotypes. At 1 ng the injections resulted in exclusively severe or lethal phenotypes. D) In vivo real time imaging of rx3:GFP embryos co-injected with nid1a and nid1b morpholinos. 3D stacks were collected every 10min for 16 h. Compared to control, nid1 morphantsdisplay a disorganized optic cup, a significant delay in the formation of opposing retinal lobes, an elongation of the N/T axis and delamination of retinal progenitor cells (yellow arrowheads). Scale bar = 50 μm.