FIGURE

Fig. 1

ID

ZDB-FIG-190806-34

Publication

Baek et al., 2019 - The Alternative Splicing Regulator Nova2 Constrains Vascular Erk Signaling to Limit Specification of the Lymphatic Lineage

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Fig. 1

nova2^uq14bh Mutants Exhibit Increased Lymphatic Sprouting

(A and B) Overall morphology of a sibling (A) and nova2^uq14bh mutant (B) at 54 hpf.

(C–F) Trunk vasculature labeled with Tg(fli1a:nEGFP)^y7;Tg(5.2lyve1b:DsRed)^nz101 in a sibling (C) and (E) and a nova2^uq14bh mutant (D) and (F) at 54 hpf (venous and LECs in red). Arrowheads indicate LEC nuclei. Venous intersegmental vessel (vISV) and PL.

(G and H) Tg(fli1ep:lifeact-EGFP) labeling endothelial F-actin in PLs of a sibling (G) and a nova2^uq14bh mutant (H) at 54 hpf. Arrowheads show exploratory PLs.

(I) EC numbers within different lineages: dorsal aorta (DA, arterial), posterior cardinal vein (PCV, venous), and lymphatic ECs (LECs) at 54 hpf. Quantification across 5 body segments (siblings n = 17, mutants n = 17).

(J) Quantification of nuclear ellipticity of ECs in the DA and PCV at 54 hpf. Quantification across 5 body segments (siblings n = 11, mutants n = 11).

(K) Quantification of hyperactive PLs (defined as displaying cellular extensions that probe dorsal to the horizontal myoseptum [HM]) in siblings and nova2^uq14bh mutants across 3 body segments (siblings n = 10, mutants n = 10).

Light gray indicates wild-type morphology, dark gray indicates exploratory PLs in 1/2 body segments scored, and black indicates exploratory PLs in 2/2 body segments scored (p = 0.0174). Error bars represent SD, t test (I), or Mann-Whitney-test (J) and (K), ^∗∗p < 0.01, ^∗∗∗∗p < 0.0001, and ns; not significant; Scale bar represents 1 mm in (A) and (B), 25 μm in (C)–(F), and 20 μm in (G) and (H).

See also Figures S1 and S2.

Expression Data

Genes:	DsRed EGFP
Fish:	nova2^uq14bh/+; nz101Tg; y7Tg nova2^{uq14bh/uq14bh}; nz101Tg; y7Tg
Anatomical Terms:	endothelial cell lymphatic system vein
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nova2^{uq14bh/uq14bh} nova2^{uq14bh/uq14bh}; nz101Tg; y7Tg
Observed In:	axis endothelial cell horizontal myoseptum vascular lymphangioblast posterior cardinal vein nucleus vascular lymphangioblast
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 49, Baek, S., Oh, T.G., Secker, G., Sutton, D.L., Okuda, K.S., Paterson, S., Bower, N.I., Toubia, J., Koltowska, K., Capon, S.J., Baillie, G.J., Simons, C., Muscat, G.E.O., Lagendijk, A.K., Smith, K.A., Harvey, N.L., Hogan, B.M., The Alternative Splicing Regulator Nova2 Constrains Vascular Erk Signaling to Limit Specification of the Lymphatic Lineage, 279-292.e5, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell