FIGURE

Figure S2

ID

ZDB-FIG-190806-15

Publication

Charlton-Perkins et al., 2019 - Genetic control of cellular morphogenesis in Müller glia

Other Figures

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Figure S2

Phenotypes of gene mutants that are enriched at specific times of MG differentiation. (a) slc45a5 controls have no observable MG phenotype. (b) timp2bmutants have defects in cell body position. (c) prmt6 mutants have defects in cell body position and ILM. (d) vwde mutants have defects in cell body position, OLM, ILM, IPL, OPL, and tiling. (e) apcdd1l mutants have defects in IPL and OPL. (f) sypb mutants have defects in OLM, OPL, and tiling. G) Cux2b mutants have defects in ILM and IPL. (h) cx31.7 mutants have defects in tiling. (i) Mpp6b mutants have defects in tiling. (j) cacnb2a mutants have defects in tiling. (h) Percentages of individual phenotypes observed in all animals from this screen. Dashed lines represent levels of significance from Fisher's exact test after Boniforni multiple test correction (bottom = p < 0.05, top = p < 0.001). Scale bars = 8μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi2001Tg + CRISPR1-apcdd1l + CRISPR2-apcdd1l mi2001Tg + CRISPR1-cux2b + CRISPR2-cux2b mi2001Tg + CRISPR1-gjb1b + CRISPR2-gjb1b mi2001Tg + CRISPR1-prmt6 + CRISPR2-prmt6 mi2001Tg + CRISPR1-seraf + CRISPR2-seraf mi2001Tg + CRISPR1-sypb + CRISPR2-sypb mi2001Tg + CRISPR2-pals2b + CRISPR3-pals2b mi2001Tg + CRISPR2-timp2b + CRISPR3-timp2b mi2001Tg + CRISPR10-cacnb2a + CRISPR1-cacnb2a
Knockdown Reagents:	CRISPR1-apcdd1l CRISPR1-cacnb2a CRISPR1-cux2b CRISPR1-gjb1b CRISPR1-prmt6 CRISPR1-seraf CRISPR1-sypb CRISPR2-apcdd1l CRISPR2-cux2b CRISPR2-gjb1b CRISPR2-pals2b CRISPR2-prmt6 CRISPR2-seraf CRISPR2-sypb CRISPR2-timp2b CRISPR3-pals2b CRISPR3-timp2b CRISPR10-cacnb2a
Observed In:	inner limiting membrane Muller cell Muller cell cell body outer limiting membrane retinal inner plexiform layer retinal outer plexiform layer
Stage:	Day 5

Phenotype Detail

Acknowledgments

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