FIGURE

Fig. 3.

ID

ZDB-FIG-190723-996

Publication

Pereira Piedade et al., 2019 - Ubiquitin-mediated proteasome degradation regulates optic fissure fusion

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3.

siah1, siah2l and nlz2 gene expression during eye morphogenesis. (A) WISH for siah1, siah2l and nlz2 between 24 and 48 hpf. (B) Two-color fluorescent WISH (FWISH) for nlz2, siah1, siah2 and pax2a. Arrowheads indicate co-localization. (C) siah1 WISH after treatment with RA (AGN194310), BMP (DMH1), hedgehog (cyclopamie) inhibitors, hedgehog agonist (purmorphamine) or in smo^hi1640Tg embryos at 24 hpf. (D) qPCR results for siah1 and pax2a expression±s.d. *P<0.05 compared to respective controls.

Expression Data

Genes:	pax2a siah1 siah2l znf503
Fish:	AB smo^{hi1640Tg/hi1640Tg}
Conditions:	chemical treatment by environment: retinoic acid receptor antagonist chemical treatment by environment: Cyclopamine chemical treatment by environment: DMH1 chemical treatment by environment: purmorphamine
Anatomical Terms:	central nervous system eye optic fissure optic fissure anatomical margin retina whole organism
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB smo^{hi1640Tg/hi1640Tg}
Conditions:	chemical treatment by environment: Cyclopamine chemical treatment by environment: DMH1 chemical treatment by environment: purmorphamine
Observed In:	central nervous system whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open