Regulation of uncx gene expression by Hedgehog (Hh) signaling pathway. Whole-mount in situ hybridization of (A–F, H–L, O, P) uncx4.1 and (G, M, N) uncx at (A, B, E, G) 19 hours post fertilization (hpf), (C, D) 24 hpf, (F) 30 hpf, (H, O, P) 34 hpf, (I, J) 14.5 hpf, and (K–N) 22 hpf. (A, C, P) Dorsal view, (B, E–O) lateral view, and (D) frontal view. (A–C, E–P) Anterior to left, and (D) toward viewer. (A, B) Spatial relationship between uncx4.1 expression and Hh signal-releasing notochord as shown by double in situ labeling with shha riboprobe at 19 hpf in (A) dorsal and (B) lateral view. No = notochord. (C, D) Loss of uncx4.1 expression in shha mRNA-injected side at 24 hpf in (C) lateral and (D) frontal view. (E–G) Expression of (E, F) uncx4.1 and (G) uncx in sonic-you (syu) mutant embryos at (E, G) 19 hpf and (F) 30 hpf (controls in Fig. 3E, 5B, and 3H, respectively). (F) Arrowheads indicate uncx4.1-expressing cells. SC = spinal cord, So = somites. (H) Expression of uncx4.1 in slow-muscle-omitted (smu) mutant embryos at 34 hpf. (I, J) Expression of uncx4.1 in (I) sibling (sib) and (J) you-too (yot) mutant embryos at 14.5 hpf. Arrowheads indicate anterior margin of expression in somites. (K–N) Expression of (K, L) uncx4.1 and (M, N) uncx in embryos treated with (K, M) EtOH and (L, N) cyclopamine A at 22 hpf in lateral view. (O, P) Expression of uncx4.1 in floating head (flh) mutant embryos at 34 hpf (controls in Fig. 3H and I, respectively).
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