FIGURE

Fig. 9

ID
ZDB-FIG-190723-1904
Publication
Mitchell et al., 2018 - Dynamic changes in microglial and macrophage characteristics during degeneration and regeneration of the zebrafish retina
Other Figures
All Figure Page
Back to All Figure Page
Fig. 9

Microglia distribute to regions containing histologically regenerated retinal neurons. AB. Images (A and B) show z projections from saline injected (AA”) or regenerated (BB”) mpeg1:GFP (green) flat-mounted whole retinas labeled with L-plastin (magenta). By 14 dpi, all immune cells in the histologically regenerated retinas express the mpeg1:GFP reporter (BB”), and the marker L-plastin can be used to label these mpeg1:GFP+ immune cells. CD Resliced images from whole flat-mounted retinas labeled with L-plastin (magenta) are shown to demonstrate distribution of microglia in control (C) and histologically regenerated retinas at 14 dpi ouabain (D). E Densities of microglia in whole, flat mounted saline injected (n = 3) and histologically regenerated retinas (14 (n = 4) or 21 dpi (n = 3) ouabain). Densities were calculated by counting microglia in individual z stacks obtained from flat mounted retinas, which included retinal layers from the ganglion cell layer to the outer nuclear layer (representing approximately 50–80 μm of depth; Fig. 7A, B in their entirety show representative regions that were quantified). Counts were normalized to 1000 μm² (area) rather than volume because regenerated retinas are thinner than controls. This area represents x and y directions parallel to the layers of the flattened retina. F Fraction of microglia in the ganglion cell layer of saline injected (n = 3) and histologically regenerated retinas (14 (n = 4) or 21 dpi (n = 3) ouabain). **p = 0.048 (two-tailed Student’s t test comparing regenerated to saline injected at indicated time point), effect size 1.56. Error bars indicate standard deviation. Scale bar in A” (applies to AA” and BB”) = 20 μm. Vertical scale bar in C and D = 20 μm

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Neuroinflammation