FIGURE

Fig. 3

ID

ZDB-FIG-190723-1310

Publication

Liu et al., 2019 - Primary cilia regulate hematopoietic stem and progenitor cell specification through Notch signaling in zebrafish

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Fig. 3

Loss of cilia genes induces hematopoietic stem and progenitor cell (HSPC) defects. a Whole-mount in situ hybridization (WISH) results of HSPC markers (runx1 and cmyb) in the aorta-gonad-mesonephros (AGM) region in control and fsd1 morphants at 36 hpf. The red arrowheads indicate the expression of HSPC markers runx1 and cmyb in DA. b Western blotting showing the protein level of Runx1 in control and fsd1 morphants at 26 hpf. c WISH analysis showing the expression of HSPC markers runx1 and cmyb (red arrowheads) in the DA in fsd1^−/−. d Western blotting showing the protein level of Runx1 in fsd1^−/−. e Representative images showing runx1 and cmyb expression in control, pkd2, kif3a, and ift88 morphants at 33 hpf. The red arrowheads indicate the expression of HSPC markers runx1 and cmyb. f WISH analysis showing the expression of HSPC marker runx1 in the DA in pkd2^−/− or ift88^−/− with a sub-effective dose of pkd2 MO (0.5 ng per embryo, pkd2 MO_low) or ift88 MO (0.8 ng per embryo, ift88 MO_low). The red arrowheads indicate the expression of HSPC marker runx1. Scale bars, 100 µm

Expression Data

Genes:	myb runx1
Fish:	AB fsd1^{ioz104/ioz104} ift88^tz288/tz288 + MO1-ift88 pkd2^{ouc2015/ouc2015} + MO3-pkd2 AB + MO1-fsd1 AB + MO1-ift88 AB + MO1-kif3a AB + MO3-pkd2
Knockdown Reagents:	MO1-fsd1 MO1-ift88 MO1-kif3a MO3-pkd2
Anatomical Terms:	ventral wall of dorsal aorta ventral wall of dorsal aorta hematopoietic multipotent progenitor cell whole organism
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fsd1^{ioz104/ioz104} ift88^tz288/tz288 + MO1-ift88 pkd2^{ouc2015/ouc2015} + MO3-pkd2 AB + MO1-fsd1 AB + MO1-ift88 AB + MO1-kif3a AB + MO3-pkd2
Knockdown Reagents:	MO1-fsd1 MO1-ift88 MO1-kif3a MO3-pkd2
Observed In:	hematopoietic progenitor cell differentiation ventral wall of dorsal aorta whole organism
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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