FIGURE

Fig 2

ID

ZDB-FIG-190723-1144

Publication

Murdoch et al., 2019 - Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization

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Fig 2

Saa regulates neutrophil abundance and maturation.

(A) Flow cytometry analysis of lyz:EGFP⁺ neutrophil abundance from whole 6 dpf WT and saa mutant zebrafish larvae (results are combined from 3 independent experiments, ≥ 4 replicates / genotype / experiment, 60–90 larvae / replicate). (B-C) Morphological analysis of lyz:DsRed⁺ neutrophil cytospins stained with Wright-Giemsa from adult WT and saa mutant zebrafish kidneys (5–6 adult zebrafish kidneys / genotype / experiment, 2 independent experiments, n ≥ 199 cells analyzed / genotype / experiment) (scale bar = 10 μm). (D-F) qRT-PCR of leukocyte-associated transcripts lysozyme C (lyz), l-plastin (lcp), and colony-stimulating factor 3a (csf3a) from 6 dpf whole zebrafish larvae (n = 4 replicates / genotype / timepoint, 25–30 larvae / replicate). Data in panel A analyzed by t-test. Data in panel C analyzed by chi-squared test. Data in panels D-F analyzed by one-way ANOVA with Tukey’s multiple comparisons test. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Expression Data

Genes:	csf3a lcp1 lyz
Fish:	saa^rdu60/rdu60
Anatomical Term:	whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	saa^rdu60/rdu60 saa^rdu60/rdu60; nz117Tg
Observed In:	immature neutrophil mature neutrophil neutrophil whole organism
Stage Range:	Day 6 to Adult

Phenotype Detail

Acknowledgments

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