Figure 7

Bioactivities of GLA tested on a zebrafish caudal fin regeneration model. (A–B) Effects of 50, 100, 250, and 500 mg L⁻¹ of GLA on zebrafish caudal fin regeneration. Proper concentrations (50 and 100 mg L⁻¹) of GLA promoted the regeneration of early fin primordia of zebrafish larvae, whereas higher concentrations (250 and 500 mg L⁻¹) did not. (C–D) Reactive oxygen species (ROS) test of wounded zebrafish treated with DMSO, 100 and 250 mg L⁻¹ of GLA. Proper concentration (100 mg L⁻¹) of GLA reduced oxidative stress at the wound region, whereas higher concentration (250 mg L⁻¹) did not. (E–F) Neutrophil attraction of wounded zebrafish caudal fin treated with DMSO and 100 mg L⁻¹ of GLA. Proper concentration (100 mg L⁻¹) of GLA reduced the number of neutrophils at the wound region revealing the anti-inflammatory effect of this fatty acid. Asterisks (*) indicate statistically significant differences compared with the DMSO (t-test, p < 0.05).