Figure 6—figure supplement 1.

Inhibiting BMP or Erbb2 signaling impairs reactivation of early cardiogenesis transcription factors.

Whole-mount in situ hybridizations show increased (B) gata4 (n = 19/25), (J) hand2 (n = 10/12) and (R) nkx2.5 (n = 12/13) expression in vmhc:mCherry-NTR ventricle-ablated hearts at 48 hpt (7 dpf) when compared to (A, I, Q) sham-ablated control hearts. However, these cardiogenesis transcription factors fail to increase in (D, L, T) dorsomorphin (DM) (n = 4/21 gata4; n = 3/12 hand2; n = 4/16 nkx2.5) and (F, N, V) AG1478-treated ventricle-ablated hearts (n = 5/20 gata4; n = 1/8 hand2; n = 3/10 nkx2.5) as well as in (H, P, X) erbb2 loss-of-function mutation (erbb2-/-) (n = 4/18 gata4; n = 5/24 hand2; n = 3/15 nkx2.5). V, ventricle; A, atrium; dpf, days post-fertilization; hpt, hours post-MTZ/DMSO treatment. Dashed lines outline the heart.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife