ARGLU1 regulates vertebrate nervous system development. (A) Mouse embryos from the Arglu1+/− x Arglu1+/− parent crosses were dissected and visually examined at E9.0 and E9.5. Arglu1+/- embryos appeared phenotypically normal whereas Arglu1−/− embryos had an overall developmental delay by approximately 0.5 days. (B) Cleaved caspase-3 was measured by immunohistochemistry in Arglu1+/+ and Arglu1−/− embryos from E9.0 and E9.5, respectively. These time points were chosen to allow cleaved caspase-3 comparisons under matched developmental stages for each genotype. (C) Zebrafish embryos were injected with the indicated morpholino antisense oligonucleotide (MO) alone or in the presence of the indicated rescue mRNA constructs and visualized at 50 hpf. Symbols represent heart edema ( ), expanded brain ventricle (*) and curved body axis ( ). (D) RNA in situ hybridization assays monitoring islet-1 expression in 50 hpf embryos. p53-MO-injected control, arglu1a morphant embryo, and arglu1a morphant embryo rescued through co-injection of arglu1a mRNA are shown. Hpf, hours post fertilization.
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