FIGURE

Fig. 2

ID

ZDB-FIG-190722-11

Publication

Stankiewicz et al., 2019 - Cell kinetics in the adult neurogenic niche and impact of diet-induced accelerated aging

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Fig. 2

The pulse-chase BrdU-EdU protocol reveals proliferating cells and newly developing neurons in young adult zebrafish brain. A, Experimental protocol involving BrdU pulse and EdU chase 1–5 d thereafter. Arrows indicate BrdU administration (red), BrdU washout (blue), EdU injection (green), and sample collection (black). BrdU and EdU exposure at ZT9–11, with ZT0 = lights-on time; 14:10 light-dark cycle. White area represents light. Gray area represents dark. N = 5 or 6 fish per time point. B, Cleared zebrafish brain following a 15 d pulse-chase. Scale bar, 150 μm. C, Labeled cells within cerebellar neurogenic niche, following 15 d chase. Arrows indicate BrdU/EdU colocalization (yellow), EdU-only (green), BrdU-only (red), and BrdU/HuC/D colocalization (magenta). Scale bar, 10 μm. D, Single-channel images for the colabeled yellow stem cell in C, BrdU (red) and EdU (green) representing NSCs. E, Single-channel images for cells colabeled for BrdU (red) and HuC/D (blue), representing immature neurons. Scale bar, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.