Fig. 1
Expression of nup133 in the developing zebrafish detected by in situ hybridization (ISH). Whole mount ISH with nup133 antisense probe of embryos at: (a) sphere stage (4 hpf; embryo shown with animal pole to the top); (b) 24 hfp (lateral view); (c–f) 3 and 5 dpf (left panels: dorsal view; right panels: lateral view). Arrows point to tissues with enriched expression of nup133. Abbreviations: E: eyes; T: tectum; C: cerebellum; L: liver; I: intestine; N: neuromasts; SB: swim bladder. Scale bars, 200 μm. (g) Transverse section of a 5 dpf embryo at the level of the pectoral fins (as shown in the dotted line in e) confirms nup133 expression in the liver, and show in addition a diffuse staining in the proximal tubules (PT) and a faint signal in the glomerulus (G). Scale bar, 50 μm. |
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Stage Range: | 1-cell to Day 5 |