FIGURE
Fig. 2
- ID
- ZDB-FIG-190703-11
- Publication
- Kuil et al., 2019 - Reverse genetic screen reveals that Il34 facilitates yolk sac macrophage distribution and seeding of the brain
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Fig. 2
SpotNGlia semi-automatically counts microglia numbers. (A) Examples of z-stack images of NR-stained larvae and a schematic representation of the SpotNGlia analysis pipeline. (B) SpotNGlia output of test dataset with both manual (blue) and automated (red) brain segmentation and NR+ microglia annotation. (C) Box plots showing Jaccard and Dice indices for accuracy of brain segmentation and F1, precision and recall scores for the accuracy of NR+microglia annotation. This is a Tukey boxplot: it uses the median and interquartile range (IQR) for the box. The whiskers extend to the most extreme data within 1.5×IQR. Data outside 1.5×IQR are considered outliers. (D) Correlation between manually and automated microglia quantification after manual correction for segmented brain area. Error bars represent s.d. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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