FIGURE

Fig. 3

ID

ZDB-FIG-190624-47

Publication

Sahu et al., 2019 - Neurotrophin receptor Ntrk2b function in the maintenance of dopamine and serotonin neurons in zebrafish

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Fig. 3

Effect of Ntrk2b deletion on dopamine and its markers at 3 dpf. (A) HPLC reveals total dopamine levels in the ntrk2b morphants at 3 dpf are reduced compared to controls. (B) (a,b) Dopaminergic neuron markers th1 and th2 transcripts were significantly reduced in the morphant. (C) (a–c) ISH of th1 revealed loss of expression in the diencephalon. This effect could be rescued with ntrk2b mRNA. (D) (a–c) The th2expression was reduced in all cell populations in the morphants. The y-axis for the Q-RT PCR represents relative expression levels normalized to b-actin. (Bars represent Mean ± SEM, Student t-test *p-value < 0.05. **p < 0.001 one-way ANOVA. Scale bar = 100 µm).

Expression Data

Genes:	th th2
Fish:	WT WT + MO2-ntrk2b
Knockdown Reagent:	MO2-ntrk2b
Anatomical Terms:	diencephalon dopaminergic neuron whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-ntrk2b
Knockdown Reagent:	MO2-ntrk2b
Observed In:	diencephalon dopaminergic neuron whole organism whole organism dopamine
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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