FIGURE

Fig. S4

ID

ZDB-FIG-190618-52

Publication

Hanovice et al., 2019 - Regeneration of the zebrafish retinal pigment epithelium after widespread genetic ablation

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

ZPR1⁺ PRs adjacent to regenerated RPE in the retinal periphery appear normal.

Transverse cryosections of unablated larvae (A,G) and ablated larvae (D,J) stained for ZPR2. Green = GFP, blue = nuclei, red = ZPR2. Magnified insets of central (B,E) and peripheral (E,F) RPE. (E-E”) At 3dpi, the ZPR1⁺ signal in PRs within the central injury site becomes less organized and reveals degeneration of outer segment tips. (F-F”) In the periphery, dim GFP signal marks the presence of regenerated RPE, and underlying ZPR1⁺ cones display morphologies similar to unablated controls. ZPR1⁺ cones closer to the periphery express normal levels of ZPR1 and identifiable outer segment tips, while those closer to the injury site display increasingly disorganized morphology and lack contiguous outer segment tips. (K-K” At 4dpi, ZPR1 signal continues to be degraded in the central injury site. (L-L”) In the periphery, where GFP⁺ RPE tissue has regenerated, ZPR1 staining is similar to unablated controls (I-I”), identifying organized cell bodies, outer segments, and outer segment tips. Scale bar = 40μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.

Fig. S4

Fig. S4

ZPR1+ PRs adjacent to regenerated RPE in the retinal periphery appear normal.

ZPR1⁺ PRs adjacent to regenerated RPE in the retinal periphery appear normal.