(A) The red dashed lines depict the respective positions of the lnc-phox2bb TSS and gene deletion. Arrows flanking black dotted line mark the primer binding sites for qRT-PCR product. (B) Homozygous gene deletion mutants but not the TSS-deletion mutants show embryonic defects in jaw formation (arrow head) and swim bladder inflation (asterisk) by 4-dpf. (C) Histone marks (H3K4me1 and H3K27ac) associated with enhancer activity (Bogdanovic et al., 2012) and conserved noncoding elements (CNEs) (Hiller et al., 2013) overlap with gene deletion. (D) phox2bb expression pattern in the TSS and gene deletions. (E) qRT-PCR analysis on MZ TSS-deletion and gene deletion mutants. The statistical significance of the observed changes was determined using t-test analysis and represented by star marks (*, **, ***, and **** respectively mark p-values<0.05,<0.01,<0.001 and<0.0001).
