Fig. 2
- ID
- ZDB-FIG-190604-52
- Publication
- Lan et al., 2019 - TETs Regulate Proepicardial Cell Migration through Extracellular Matrix Organization during Zebrafish Cardiogenesis
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Epicardial Cells from Donor Hearts Do Not Migrate onto tet2/3DM Recipient Hearts (A) Schematic of epicardial cell migration assay. Wild-typetcf21:DsRed donor hearts (isolated at 72 hpf) were co-cultured with either wild-type or tet2/3DM myl7:GFP recipient hearts (isolated at 48 hpf) for one week and then confocal images taken. (B) Confocal images showed epicardial cells from wild-type donor heart can migrate onto wild-type recipient hearts. In 30 pairs of co-cultured hearts, 6 of recipient hearts were observed having epicardial cells migrated from donor hearts. (C) Confocal images showed epicardial cells from wild-type donor heart failed to migrate onto tet2/3DM recipient hearts. In 60 pairs of co-cultured hearts, none of the recipient hearts were observed having epicardial cells from donor hearts. Scale bar: 50 μm. |