FIGURE
Fig. 4
- ID
- ZDB-FIG-190604-36
- Publication
- DeLaurier et al., 2019 - hdac4 mediates perichondral ossification and pharyngeal skeleton development in the zebrafish
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Fig. 4
Expression of runx2a, runx2b, and sp7 mRNA in the pharyngeal skeleton of wild-type and mutant larvae at 4 dpf, detected using mRNA in situ hybridization using the hdac4aik3line.(A, C, E, G, I, K) Schematic views of skeletal elements, ventral view, blue indicates cartilage, red indicates bone. (B and D) expression of runx2a in wild-type (B) and mutant (D), arrows indicate expression at the posterior margin of the ceratohyal. (F and H) expression of runx2bin wild-type (F) and mutant (H), arrows indicate expression at the posterior margin of the ceratohyal. (J and L) expression of sp7 in wild-type (J) and mutant (L), arrows indicate expression at the posterior margin of the ceratohyal. M and N, schematic showing overlapping domains of expression of runx2a, runx2b, and sp7 in wild-type (M) and mutant (N) larvae. (O–Q) Average length of expression, bars in graph represent means and error bars are standard errors of means (SEM); runx2a (wild-type n = 10, mean = 52.9, SEM = 2.86; mutant n = 11, mean = 61.55, SEM = 1.95), runx2b (wild-type n = 8, mean = 30.33, SEM = 1.34; mutant n = 9, mean = 35.65, SEM = 0.82), sp7 (wild-type n = 12, mean = 21.73, SEM = 3.65; mutant n = 10, mean = 21.78, SEM = 3.15). Abbreviations: ch, ceratohyal; bsr, branchiostegal ray; pa3, pharyngeal arch 3; pq, palatoquadrate. *p ≤ 0.05, **p ≤ 0.01. B, D, scale bar = 50 microns, F, H, J, L, scale bar = 25 microns. Note that anterior and posterior lengths were at measured at different positions within the z-stack, and not all maximum lengths (anterior, posterior, right, and left ceratohyal) are represented in the sections shown in B, D, F, and H. |
Expression Data
| Genes: | |
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| Fish: | |
| Anatomical Terms: | |
| Stage: | Day 4 |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
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| Observed In: | |
| Stage: | Day 4 |
Phenotype Detail
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