FIGURE

Fig. 5

ID

ZDB-FIG-190528-4

Publication

Bose et al., 2018 - Neuromuscular junction abnormalities in a zebrafish loss-of-function model of TDP-43

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Significantly compromised neuromuscular junction (NMJ) connectivity in hom/hom mutants. A: representative images of one ventral root projection at 2 days postfertilization (dpf) labeled with synaptotagmin 1 (ZNP-1; presynaptic marker), sulforhodamine-conjugated α-bungarotoxin (αBTX; postsynaptic marker), and merged. B: representative images of one ventral root projection at 3 dpf labeled with ZNP-1 (presynaptic marker), sulforhodamine-conjugated αBTX (postsynaptic marker), and merged. Wild-type (WT) larvae demonstrate tight colocalization of both ZNP-1 and αBTX (merged). Scale bar is 25 μm for all images. C: quantification of orphaned ZNP-1 puncta to total ZNP-1 puncta. At 2 dpf, all other genotypes displayed significantly greater orphaned ZNP-1 puncta compared with WT larvae (one-way ANOVA, post hoc Tukey’s test; #P < 0.05). At 3dpf, all other genotypes displayed significantly greater orphaned ZNP-1 puncta compared with WT larvae (one-way ANOVA, post hoc Tukey’s test; $P < 0.05). Paired t-test between the same group at 2 and 3 dpf indicates a significant difference in het/hom and hom/hom mutants (paired t-test; *P < 0.05; **P < 0.01). D: quantification of orphaned αBTX puncta to total αBTX puncta. At 2 dpf, all other genotypes displayed significantly greater orphaned ZNP-1 puncta compared with WT larvae (one-way ANOVA, post hoc Tukey’s test; #P < 0.05). At 3dpf, all other genotypes displayed significantly greater orphaned ZNP-1 puncta compared with WT larvae (one-way ANOVA, post hoc Tukey’s test; $P < 0.05). Paired t-test between the same group at 2 and 3 dpf indicates a significant difference in tardbp^−/− and hom/hom mutants (paired t-test; *P < 0.05; **P < 0.01). Data are means ± SE. Numbers in parentheses indicate sample sizes.

Expression Data

Gene:	chrna1
Antibody:	znp-1
Fish:	WT tardbpa^{udm104/udm104} tardbpb^fh301/fh301 tardbpb^fh301/+; tardbpa^{udm104/udm104} tardbpb^fh301/fh301; tardbpa^{udm104/udm104}
Anatomical Terms:	fast muscle cell postsynapse of neuromuscular junction motor neuron terminal bouton
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tardbpa^{udm104/udm104} tardbpb^fh301/fh301 tardbpb^fh301/+; tardbpa^{udm104/udm104} tardbpb^fh301/fh301; tardbpa^{udm104/udm104}
Observed In:	myotome neuromuscular junction development
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurophysiol.