FIGURE

Fig. 4

ID

ZDB-FIG-190328-4

Publication

Chambers et al., 2018 - ppargc1a controls nephron segmentation during zebrafish embryonic kidney ontogeny

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

ppargc1a acts upstream of tbx2b to form the DL segment. (A) WISH for tbx2b (purple) expression in 28 ss WT and ppargc1asa13186-/-zebrafish. Scale bars = 75 μm. (B) Absolute length measurements of tbx2b mRNA expression domains in WT and ppargc1asa13186-/- zebrafish. (C) ppargc1a expression in WT and tbx2b MO injected 28 ss zebrafish. Scale bars = 65 μm. (D) Absolute length measurements of ppargc1a expression domain in WT and tbx2b MO injected zebrafish. (E) WISH at 28 ss for slc12a3 (purple) in WT, ppargc1asa13186-/-, and ppargc1asa13186-/- injected with tbx2b cRNA. Scale bars = 75 μm. (F) Absolute length measurements of slc12a3 mRNA expression domains in WT, ppargc1asa13186-/-, and ppargc1asa13186-/- + tbx2b cRNA. Data are represented as ±SD, significant by t test, n.s. = not significant, ** = p < 0.01, and *** = p < 0.001.

Expression Data

Genes:	ppargc1a slc12a3 tbx2b
Fish:	TU ppargc1a^{sa13186/sa13186} TU + MO1-tbx2b
Knockdown Reagent:	MO1-tbx2b
Anatomical Terms:	pronephric distal late tubule pronephric duct
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ppargc1a^{sa13186/sa13186}
Observed In:	pronephric distal late tubule pronephric duct
Stage:	26+ somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife