FIGURE

Fig. 3

ID

ZDB-FIG-190308-9

Publication

Burg et al., 2018 - Conditional mutagenesis by oligonucleotide-mediated integration of loxP sites in zebrafish

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Fig. 3

Conversion of a Cre-revertible Gene Breaking Transposon (GBT) allele to a conditional allele. a. Diagram of the fleer locus. Exons drawn to scale, introns not to scale. Below each intron-exon junction reading frame phase is indicated. b. Diagram of the fleer gene trap allele flrtpl19. c. Diagram flrtpl19R locus reverted by Cre-mediated excision of the gene trap cassette. d. Diagram of flr sgRNA3 target site and antisense oligonucleotide HDR template. e. Sequence of loxP integration into intron 7, resulting in floxed fleer allele tpl141. f, g, h. Cre-mediated excision of exons 2–7 of fleer. f. Induction of fleer deletion by Cre mRNA. g. One quarter of embryos obtained by in-crossing fleertpl141 heterozygotes and injected with Cre mRNA display a phenotype consistent with fleertpl19 homozygotes in h, including formation of kidney cysts shown in g’ and h’. i. Sequence of the excision amplicon.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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