FIGURE

Fig. 3

ID

ZDB-FIG-190201-29

Publication

Sokol et al., 2018 - Loss of the Mia40a oxidoreductase leads to hepato-pancreatic insufficiency in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Abnormal mitochondrial structures are found in the skeletal muscles of mia40a mutants.

(A) Embryos obtained from an in-cross of heterozygous mia40a^+/- siblings in the Tg(Xla.Eef1a1:mlsEGFP) background were stained with phalloidin to visualize actin filaments of the skeletal muscle at 4 dpf. Abnormal, enlarged mitochondrial structures are found in the skeletal muscles of the mia40a mutants (arrowheads). To better visualize the GFP-positive inclusions, a magnified image is shown on the side. Images are maximum projections. Scale bar, 50 μm. All images are lateral views, anterior to the left. (B) wild-type and mia40a^-/- larvae in the Tg(Xla.Eef1a1:mlsEGFP) background were stained with an anti-Tomm20 antibody to mark the outer mitochondrial membrane (OMM). DNA was counterstained with DAPI. Single plane sections are shown. The arrowhead points to a GFP-positive inclusion. Scale bar, 5 μm. All images are lateral views, anterior to the left. (C) wild-type or mia40a^-/- siblings in AB background were subjected to transmission electron microscopy (TEM) analysis at 5 dpf. Arrowheads point to well defined mitochondrial membranes in wild-type larvae. Abnormal membranous structures found in the mia40a mutants are magnified (inset). Scale bar, 500 nm. M: Mitochondria.

Expression Data

Gene:	EGFP
Antibody:	Ab1-tomm20
Fish:	cms1Tg chchd4a^bns292/+; cms1Tg chchd4a^{bns292/bns292}; cms1Tg
Anatomical Terms:	skeletal muscle skeletal muscle mitochondrial matrix skeletal muscle mitochondrial outer membrane
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	chchd4a^{bns292/bns292} (AB) chchd4a^{bns292/bns292}; cms1Tg
Observed In:	skeletal muscle skeletal muscle inclusion body skeletal muscle mitochondrion
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.